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ISO 23893-3

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ISO 23893-3 1st Edition, April 1, 2013 Water quality - Biochemical and physiological measurements on fish - Part 3: Determination of vitellogenin

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Description / Abstract: This part of ISO 23893 specifies a method for measuring vitellogenin (Vtg) concentrations in a fish plasma sample employing an enzyme-linked immunosorbent assay (ELISA) method.

It applies to fish that are sampled in the environment (fresh, estuarine or salt water) and to fish exposed to substances or effluents in a laboratory. The method is quantitative when using Vtg antibodies and a Vtg standard well characterized with the species of choice.