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ISO TS 15216-2 1st Edition, March 15, 2013 Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection
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Availability date: 07/13/2021
Description / Abstract:
This part of ISO/TS 15216 describes a method for qualitative
detection of HAV and NoV genogroups I (GI) and II (GII), from test
samples of foodstuffs or food surfaces. Following liberation of
viruses from the test sample, viral RNA is then extracted by lysis
with guanidine thiocyanate and adsorption on silica. Target
sequences within the viral RNA are amplified and detected by
real-time RT-PCR.
This approach is also relevant for detection of the target
viruses on fomites, or of other human viruses in foodstuffs, on
food surfaces or on fomites following appropriate validation and
using target-specific primer and probe sets.